Journal: bioRxiv
Article Title: Neurovascular mitochondrial susceptibility impacts blood-brain barrier function and behavior
doi: 10.1101/2024.02.15.580544
Figure Lengend Snippet: ( A ) Representative plot of oxygen consumption rate by a bezafibrate-treated and untreated 22qDS iBBB line during the Seahorse XFe96 Analyzer mitochondrial stress test ( n = 4 22qDS lines, 7 replicates per condition). ( B ) Quantification of OXPHOS parameters for each 22qDS line comparing bezafibrate-treated and untreated conditions ( n = 4 22qDS lines, 7 replicates per condition, paired t test). ( C ) Schematic is describing key regulators of mitochondrial dynamics. ( D ) Quantification of gene transcripts for fusion and fission ( n = 4 22qDS lines, paired t test). ( E ) Representative time course of TEER fold change following bezafibrate treatment in a 22qDS iBBB line, normalized to untreated ( n = 6-11 replicates per condition, two-way ANOVA). ( F ) Quantification of TEER at 36 hours following bezafibrate treatment ( n = 4 22qDS lines, 3-4 replicates per condition – 2 experiments per line, paired t test). ( G ) Immunofluorescence of claudin-5 (green) in 22qDS in untreated (top) compared to bezafibrate treatment (bottom). Error bars ± SEM. Scale bar: 10 μm.
Article Snippet: Primary antibody rabbit polyclonal antibodies against claudin-5 (Life Technologies) was diluted 1:300 in 3% normal donkey serum and incubated overnight at 4°C.
Techniques: Immunofluorescence